Journal: bioRxiv

Article Title: Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila

doi: 10.1101/2023.04.14.536839

Figure Lengend Snippet: A , number of NB II-derived (GFP-positive) cells per larva (based on 3-7 independent dissections per genotype, each involving 130-240 larvae). B , NB II lineages in brains from 3rd instar larvae were marked with mCD8::GFP (green) and co-stained for the nuclear proteins Dpn (red) and the apoptosis marker Dcp-1 (blue). Few apoptotic cells are observed in control, Spt5-KD and brat-KD brains but their number increases under double-knockdown conditions. Scale bar: 10 µm.

Article Snippet: After blocking in PBT containing 5% normal goat serum for 1 h, tissues were incubated overnight at 4°C with combinations of the following primary antibodies: rabbit anti-Ase (1:400; F. Diaz-Benjumea, Madrid, Spain), mouse anti-Bruchpilot (1:30, clone nc82; E. Buchner, Würzburg, Germany), rabbit anti-Dcp-1 (1:100, Cell Signaling Techn.

Techniques: Derivative Assay, Staining, Marker

Journal: eLife

Article Title: A neurotrophin functioning with a Toll regulates structural plasticity in a dopaminergic circuit

doi: 10.7554/eLife.102222

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-DCP-1 , Cell Signalling , Cat# 8578S , 1:250.

Techniques: CRISPR, Reverse Transcription, Plasmid Preparation, Software